Interleukin enhancer-binding factor 3 (ILF3) (N-Term) antibody

Catalog No. ABIN2780867

This Rabbit Polyclonal antibody specifically detects Interleukin enhancer-binding factor 3 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse and Zebrafish (Danio rerio).

Quick Overview for Interleukin enhancer-binding factor 3 (ILF3) (N-Term) antibody (ABIN2780867)

Target: Interleukin enhancer-binding factor 3 (ILF3)

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details

Binding Specificity: N-Term

Purpose: ILF3 Antibody - N-terminal region

Sequence: IFVNDDRHVM AKHSSVYPTQ EELEAVQNMV SHTERALKAV SDWIDEQEKG

Specificity: The immunizing peptide used to raise this antibody is 100 % homologous to isoform 2 (702aa, 76 kDa), 3 (764aa, 83 kDa), 4 (698aa, 76 kDa) and 5 (690aa, 75 kDa), 6 (706aa, 77 kDa) and 7 (898aa, 96 kDa) of human ILF3.

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against ILF3. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ILF3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8103403

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: Interleukin enhancer-binding factor 3 (ILF3)

Alternative Name: ILF3

Background:

Background Information: Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of ILF3.Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.

Gene Name: Interleukin enhancer binding factor 3, 90 kDa

Alternative Symbols: CBTF, DRBF, MMP4, MPP4, NF90, NFAR, NF110, NF90a, NF90b, NF90c, NFAR2, TCP80, DRBP76, NF110b, NFAR-1, NFAR-2, NFAR90, TCP110, MPP4110, NF90ctv, NFAR110, MPHOSPH4, NF-AT-90

Protein Name: Interleukin enhancer-binding factor 3

Molecular Weight: 95kDa

Gene ID: 3609

NCBI Accession: NP_004507

UniProt: Q12906

Pathways: M Phase