SETDB1 antibody (N-Term)

Catalog No. ABIN2780883

This Rabbit Polyclonal antibody specifically detects SETDB1 in WB. It exhibits reactivity toward Human, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for SETDB1 antibody (N-Term) (ABIN2780883)

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Reactivity: Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETDB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SETDB1 Antibody

Binding Specificity: N-Term

Sequence: PGCIGLDAAT ATVESEEIAE LQQAVVEELG ISMEELRHFI DEELEKMDCV

Predicted Reactivity: Cow: 92%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Rabbit: 85%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against SETDB1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human SETDB1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SETDB1

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Alternative Name: SETDB1

Background: SETDB1 is a histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
Protein Interaction Partner: MDM2, HSP90AA1, UBC, SKIL, LRIF1, BARD1, APLP1, TCERG1, TRIM28, VIM, SUMO2, PML, CLSTN1, JARID2, tat, MZT2B, FAM118B, MRPL44, LUC7L2, MAP4K5, TSC22D1, TTR, TK1, SMN1, PCYT2, PSME1, GRB7, CDKN1A, CDK4, BID, ANXA7, ZNF274, HIST1H3A, ESCO1, AKT2, AKT1, PPARG
Protein Size: 397

Molecular Weight: 43 kDa

Gene ID: 9869