CNOT7 antibody (N-Term)

Catalog No. ABIN2780900

The Rabbit Polyclonal anti-CNOT7 antibody has been validated for WB and IHC. It is suitable to detect CNOT7 in samples from Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Horse, Rabbit, Guinea Pig, Goat and Saccharomyces cerevisiae. There are 2+ publications available.

Quick Overview for CNOT7 antibody (N-Term) (ABIN2780900)

Target: CNOT7 (CCR4-NOT Transcription Complex, Subunit 7 (CNOT7))

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Horse, Rabbit, Guinea Pig, Goat, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNOT7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CNOT7 Antibody

Binding Specificity: N-Term

Sequence: TEFPGVVARP IGEFRSNADY QYQLLRCNVD LLKIIQLGLT FMNEQGEYPP

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 79%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against CNOT7. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CNOT7

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 262 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CNOT7 antibody (ABIN2780900)

Maragozidis, Karangeli, Labrou, Dimoulou, Papaspyrou, Salataj, Pournaras, Matsouka, Gourgoulianis, Balatsos: "Alterations of deadenylase expression in acute leukemias: evidence for poly(a)-specific ribonuclease as a potential biomarker." in: Acta haematologica, Vol. 128, Issue 1, pp. 39-46, (2012) (PubMed).

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for CNOT7

Target: CNOT7 (CCR4-NOT Transcription Complex, Subunit 7 (CNOT7))

Alternative Name: CNOT7

Background: CNOT7 binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The protein has both mouse and yeast orthologs.The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The protein has both mouse and yeast orthologs. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Alias Symbols: CAF1, hCAF-1
Protein Interaction Partner: FBXW11, TOB2, BTG2, FOXC2, UBC, BAG3, CNOT6, AGO2, AGO1, EPAS1, APP, ELAVL1, BTG1, Cnot3, HOXD4, IKBKG, BTG3, TOB1, CDK6, CDK4, CDK2, CDK1, PABPC1,
Protein Size: 262

Molecular Weight: 30 kDa

Gene ID: 29883

NCBI Accession: NM_013354, NP_037486

UniProt: Q9UIV1

Pathways: Ribonucleoprotein Complex Subunit Organization