SART3 antibody (N-Term)

Catalog No. ABIN2780944

This Rabbit Polyclonal antibody specifically detects SART3 in WB and IHC. It exhibits reactivity toward Human, Rat, Guinea Pig, Dog, Horse and Pigand has been mentioned in 1 publication.

Quick Overview for SART3 antibody (N-Term) (ABIN2780944)

Target: SART3 (Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))

Reactivity: Human, Rat, Guinea Pig, Dog, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SART3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SART3 Antibody

Binding Specificity: N-Term

Sequence: TSASEPEAES KAGPKADGEE DEVKAARTRR KVLSRAVAAA TYKTMGPAWD

Predicted Reactivity: Dog: 77%, Guinea Pig: 79%, Horse: 79%, Human: 100%, Pig: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against SART3. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SART3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 364 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SART3 antibody (ABIN2780944)

Kaji, Mizukoshi, Yamashita, Arai, Sunagozaka, Fushimi, Nakagawa, Yamada, Terashima, Kitahara, Kaneko: "Cellular Immune Responses for Squamous Cell Carcinoma Antigen Recognized by T Cells 3 in Patients with Hepatocellular Carcinoma." in: PLoS ONE, Vol. 12, Issue 1, pp. e0170291, (2017) (PubMed).

Target Details for SART3

Target: SART3 (Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))

Alternative Name: SART3

Background: SART3 is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. SART3 is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This protein is thought to be involved in the regulation of mRNA splicing.
Alias Symbols: P100, p110, DSAP1, TIP110, p110(nrb), RP11-13G14
Protein Interaction Partner: APBB1, PRPF3, Usp15, AMOT, SF3B1, NUP160, LRPPRC, THRAP3, BCLAF1, VARS, UBC, SUMO2, RAB1A, NEFM, IARS, HIF1A, DSG2, DHX9, MEPCE, HIST2H2BE, HIST2H2AC, RNF2, BMI1, RUFY1, NRBP1, PSME4, AKAP2, COPS5, NUP50, POP7, ARHGEF10, PSMB3, PSMA5, PSMA3, POLR2H, CAST,
Protein Size: 364

Molecular Weight: 40 kDa

Gene ID: 9733

NCBI Accession: NM_014706

Pathways: Ribonucleoprotein Complex Subunit Organization