UBR1 antibody (N-Term)

Catalog No. ABIN2781277

This Rabbit Polyclonal antibody specifically detects UBR1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig and Saccharomyces cerevisiaeand has been mentioned in 1 publication.

Quick Overview for UBR1 antibody (N-Term) (ABIN2781277)

Target: UBR1 (Ubiquitin Protein Ligase E3 Component N-Recognin 1 (UBR1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBR1 Antibody

Binding Specificity: N-Term

Sequence: YKQLQKEYIS DDHDRSISIT ALSVQMFTVP TLARHLIEEQ NVISVITETL

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Yeast: 82%

Characteristics: This is a rabbit polyclonal antibody against UBR1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBR1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1749 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-UBR1 antibody (ABIN2781277)

Yamaguchi, Moritani, Tanahashi, Osabe, Nomura, Fujita, Keshavarz, Kunika, Nakamura, Yoshikawa, Ichiishi, Shiota, Yasui, Inoue, Itakura: "Lack of association of genetic variation in chromosome region 15q14-22.1 with type 2 diabetes in a Japanese population." in: BMC medical genetics, Vol. 9, pp. 22, (2008) (PubMed).

Target Details for UBR1

Target: UBR1 (Ubiquitin Protein Ligase E3 Component N-Recognin 1 (UBR1))

Alternative Name: UBR1

Background: UBR1 is an E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. UBR1 may be involved in pancreatic homeostasis.The N-end rule pathway is one proteolytic pathway of the ubiquitin system. The recognition component of this pathway, encoded by this gene, binds to a destabilizing N-terminal residue of a substrate protein and participates in the formation of a substrate-linked multiubiquitin chain. This leads to the eventual degradation of the substrate protein. The protein described in this record has a RING-type zinc finger and a UBR-type zinc finger. Mutations in this gene have been associated with Johanson-Blizzard syndrome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: JBS, MGC142065, MGC142067
Protein Interaction Partner: CDC6, UBC, SHFM1, UBE2D1, CBX8, ZNF593, DNPH1, USP5, XPO1, PFAS, CSE1L, ATP6V1A, VCL, UCHL3, CUL2, RECQL4, TIRAP, DNAJB11, NUDT3, SPRY2, LAMTOR3, UBE2A, GSK3B, GADD45A, BCL2L1, UBE2Z, NSFL1C, UBXN1, UBXN7, FAF2, FAF1, FOS, HNRNPA1, NDUFA4L2, ARL15, BTBD2,
Protein Size: 1749

Molecular Weight: 200 kDa

Gene ID: 197131

NCBI Accession: NM_174916, NP_777576

UniProt: Q8IWV7