HECTD2 antibody (C-Term)

Catalog No. ABIN405565

This Rabbit Polyclonal antibody specifically detects HECTD2 in WB. It exhibits reactivity toward Human, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Rat, Saccharomyces cerevisiae and Zebrafish (Danio rerio)and has been mentioned in 1 publication.

Quick Overview for HECTD2 antibody (C-Term) (ABIN405565)

Target: HECTD2 (HECT Domain Containing 2 (HECTD2))

Reactivity: Human, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Rat, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HECTD2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HECTD2 Antibody

Binding Specificity: C-Term

Sequence: TDLTIKYFWD VVLGFPLDLQ KKLLHFTTGS DRVPVGGMAD LNFKISKNET

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 86%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against HECTD2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HECTD2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 776 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HECTD2 antibody (ABIN405565)

Sun, Wang, He, Sweeney, Liu, Brown, Balk, Lee, Kantoff: "MiR-221 promotes the development of androgen independence in prostate cancer cells via downregulation of HECTD2 and RAB1A." in: Oncogene, Vol. 33, Issue 21, pp. 2790-800, (2014) (PubMed).

Target Details for HECTD2

Target: HECTD2 (HECT Domain Containing 2 (HECTD2))

Alternative Name: HECTD2

Background: HECTD2 is a probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
Alias Symbols: FLJ16050
Protein Interaction Partner: ELAVL1, UBC,
Protein Size: 776

Molecular Weight: 88 kDa

Gene ID: 143279

NCBI Accession: NM_182765, NP_877497

UniProt: Q5RD78