HECTD2 antibody (C-Term)

Catalog No. ABIN2781287

This Rabbit Polyclonal antibody specifically detects HECTD2 in WB. It exhibits reactivity toward Human, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Rat, Saccharomyces cerevisiae and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for HECTD2 antibody (C-Term) (ABIN2781287)

Target: HECTD2 (HECT Domain Containing 2 (HECTD2))

Reactivity: Human, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Rat, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HECTD2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HECTD2 Antibody

Binding Specificity: C-Term

Purpose: HECTD2 Antibody - C-terminal region

Sequence: TDLTIKYFWD VVLGFPLDLQ KKLLHFTTGS DRVPVGGMAD LNFKISKNET

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 86%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against HECTD2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HECTD2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43481-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-HECTD2 antibody (ABIN2781287)

Sun, Wang, He, Sweeney, Liu, Brown, Balk, Lee, Kantoff: "MiR-221 promotes the development of androgen independence in prostate cancer cells via downregulation of HECTD2 and RAB1A." in: Oncogene, Vol. 33, Issue 21, pp. 2790-800, (2014) (PubMed).

Target Details for HECTD2

Target: HECTD2 (HECT Domain Containing 2 (HECTD2))

Alternative Name: HECTD2

Background:

Background Information: HECTD2 is a probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.

Gene Name: HECT domain containing E3 ubiquitin protein ligase 2

Alternative Symbols: FLJ16050

Protein Name: Probable E3 ubiquitin-protein ligase HECTD2

Molecular Weight: 88kDa

Gene ID: 143279

NCBI Accession: NP_877497

UniProt: Q5RD78