ABCC8 antibody (N-Term)

Catalog No. ABIN2781496

Cited in 1 publication. The Rabbit Polyclonal anti-ABCC8 antibody (ABIN2781496) specifically detects ABCC8 in WB and IHC. The antibody is reactive with Human, Rat, Mouse, Dog, Cow, Pig, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for ABCC8 antibody (N-Term) (ABIN2781496)

Target: ABCC8 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8))

Reactivity: Human, Rat, Mouse, Dog, Cow, Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCC8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ABCC8 Antibody

Binding Specificity: N-Term

Sequence: PLAFCGSENH SAAYRVDQGV LNNGCFVDAL NVVPHVFLLF ITFPILFIGW

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against ABCC8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ABCC8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1581 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ABCC8 antibody (ABIN2781496)

Němcová-Fürstová, Kopperová, Balušíková, Ehrlichová, Brynychová, Václavíková, Daniel, Souček, Kovář: "Characterization of acquired paclitaxel resistance of breast cancer cells and involvement of ABC transporters." in: Toxicology and applied pharmacology, Vol. 310, pp. 215-228, (2016) (PubMed).

Target Details for ABCC8

Target: ABCC8 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8))

Alternative Name: ABCC8

Background: ABCC8 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). ABCC8 is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion.The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternative splicing of this gene has been observed, however, the transcript variants have not been fully described. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: ABC36, HHF1, HI, HRINS, MRP8, PHHI, SUR, SUR1, TNDM2, SUR1delta2
Protein Interaction Partner: ENSA, KCNJ11, CRYBB1, KCNJ8, RAPGEF4,
Protein Size: 1581

Molecular Weight: 177 kDa

Gene ID: 6833

NCBI Accession: NM_000352, NP_000343

UniProt: Q09428

Pathways: Negative Regulation of Hormone Secretion