GLUT4 antibody (N-Term)

Catalog No. ABIN2781558

This Rabbit Polyclonal antibody specifically detects GLUT4 in WB. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Dog, Cow, Goat, Horse, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for GLUT4 antibody (N-Term) (ABIN2781558)

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Goat, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUT4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GLUT4 Antibody

Binding Specificity: N-Term

Sequence: LQFGYNIGVI NAPQKVIEQS YNETWLGRQG PEGPSSIPPG TLTTLWALSV

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against SLC2A4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SLC2A4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 509 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GLUT4

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Alternative Name: SLC2A4

Background: SLC2A4 is a member of the solute carrier family 2 (facilitated glucose transporter) family and functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus.This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: GLUT4
Protein Interaction Partner: UBC, FAR1, P3H1, FKBP10, ESYT2, GNL3L, CRNKL1, STRN3, ARFIP1, SDF2L1, P3H4, KLHL41, PDIA6, MVP, RAB7A, UBE2N, PSMD7, PRKAR1A, NSF, IDH1, HSP90AA1, HK2, GAPDH, DLD, CTSD, SERPINH1, BSG, ARF5, ARF3, ACTN1, ACTN4, ACOX1, DSK2, UBI4, C1QTNF5, MAP1S, EHD2, UBE
Protein Size: 509

Molecular Weight: 55 kDa

Gene ID: 6517

NCBI Accession: NM_001042, NP_001033

UniProt: P14672

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Proton Transport, Brown Fat Cell Differentiation, Warburg Effect