ATP4b antibody (Middle Region)

Catalog No. ABIN2781819

The Rabbit Polyclonal anti-ATP4b antibody (ABIN2781819) specifically detects ATP4b in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for ATP4b antibody (Middle Region) (ABIN2781819)

Target: ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP4b antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP4b Antibody

Binding Specificity: Middle Region

Sequence: QVKYYPPNGT FSLHYFPYYG KKAQPHYSNP LVAAKLLNIP RNAEVAIVCK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against ATP4B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATP4B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 291 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATP4b

Target: ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))

Alternative Name: ATP4B

Background: ATP4B belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: ATP6B
Protein Interaction Partner: CCNDBP1, ATP4A, AGO3, UBC, BPGM, STX1A,
Protein Size: 291

Molecular Weight: 33 kDa

Gene ID: 496

NCBI Accession: NM_000705, NP_000696

UniProt: P51164

Pathways: Proton Transport