B4GALNT1 antibody (Middle Region)

Catalog No. ABIN2782130

The Rabbit Polyclonal anti-B4GALNT1 antibody has been validated for WB. It is suitable to detect B4GALNT1 in samples from Human, Mouse, Rat, Cow, Dog, Pig, Rabbit and Guinea Pig. There is 1 publication available.

Quick Overview for B4GALNT1 antibody (Middle Region) (ABIN2782130)

Target: B4GALNT1 (beta-1,4-N-Acetyl-Galactosaminyl Transferase 1 (B4GALNT1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B4GALNT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-B4GALNT1 Antibody

Binding Specificity: Middle Region

Sequence: GLGSLRVGSC SDVVVDHASK LKLPWTSRDA GAETYARYRY PGSLDESQMA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against B4GALNT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human B4GALNT1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 533 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-B4GALNT1 antibody (ABIN2782130)

Trilck, Peter, Zheng, Frank, Dobrenis, Mascher, Rolfs, Frech: "Diversity of glycosphingolipid GM2 and cholesterol accumulation in NPC1 patient-specific iPSC-derived neurons." in: Brain research, Vol. 1657, pp. 52-61, (2016) (PubMed).

Target Details for B4GALNT1

Target: B4GALNT1 (beta-1,4-N-Acetyl-Galactosaminyl Transferase 1 (B4GALNT1))

Alternative Name: B4GALNT1

Background: GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. B4GALNT1(GalNAc-T) catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively.
Alias Symbols: GALGT, GALNACT
Protein Interaction Partner: UBC, B4GALNT1,
Protein Size: 533

Molecular Weight: 59 kDa

Gene ID: 2583

NCBI Accession: NM_001478, NP_001469

UniProt: Q00973