KLKB1 antibody (Middle Region)

Catalog No. ABIN2782312

This Rabbit Polyclonal antibody specifically detects KLKB1 in WB. It exhibits reactivity toward Human, Rat, Dog, Horse and Pigand has been mentioned in 1 publication.

Quick Overview for KLKB1 antibody (Middle Region) (ABIN2782312)

Target: KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))

Reactivity: Human, Rat, Dog, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLKB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KLKB1 Antibody

Binding Specificity: Middle Region

Sequence: VLTAAHCFDG LPLQDVWRIY SGILNLSDIT KDTPFSQIKE IIIHQNYKVS

Predicted Reactivity: Dog: 86%, Horse: 86%, Human: 100%, Pig: 79%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against KLKB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human KLKB1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 638 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-KLKB1 antibody (ABIN2782312)

Tang, Yu, Williams, Springman, Jeffery, Sprengeler, Estevez, Sampang, Shrader, Spencer, Young, McGrath, Katz: "Expression, crystallization, and three-dimensional structure of the catalytic domain of human plasma kallikrein." in: The Journal of biological chemistry, Vol. 280, Issue 49, pp. 41077-89, (2005) (PubMed).

Target Details for KLKB1

Target: KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))

Alternative Name: KLKB1

Background: Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients.Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients.
Alias Symbols: KLK3, PPK
Protein Interaction Partner: TFPI2, HGFAC, SERPINA5, F12, KNG1, IGFBP3, HGF, MST1,
Protein Size: 638

Molecular Weight: 28 kDa

Gene ID: 3818

NCBI Accession: NM_000892, NP_000883

UniProt: P03952

Pathways: Complement System, Brown Fat Cell Differentiation