CSTB antibody (Middle Region)

Catalog No. ABIN2782349

The Rabbit Polyclonal anti-CSTB antibody has been validated for WB and IHC. It is suitable to detect CSTB in samples from Human, Rat, Mouse, Horse, Pig, Rabbit and Dog.

Quick Overview for CSTB antibody (Middle Region) (ABIN2782349)

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Reactivity: Human, Rat, Mouse, Horse, Pig, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSTB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CSTB Antibody

Binding Specificity: Middle Region

Sequence: AGTNYFIKVH VGDEDFVHLR VFQSLPHENK PLTLSNYQTN KAKHDELTYF

Predicted Reactivity: Dog: 92%, Horse: 85%, Human: 100%, Mouse: 83%, Pig: 79%, Rabbit: 77%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against CSTB. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CSTB

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 98 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CSTB

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Alternative Name: CSTB

Background: CSTB is a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in CSTB gene are responsible for the primary defects in patients with progressive myoclonic epilepsy a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy.The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1).
Alias Symbols: CST6, EPM1, PME, STFB, ULD, EPM1A
Protein Interaction Partner: UBC, SARNP, DPP7, BAG3, CFTR, ECT2, SEC24A, NUDC, MAT2A, FLNB, DCUN1D1, CUL3, RAD21, SPRY2, CTSL, CTSD, CTSH, CTSB, CST3, VHL,
Protein Size: 98

Molecular Weight: 11 kDa

Gene ID: 1476

NCBI Accession: NM_000100, NP_000091

UniProt: P04080

Pathways: Response to Water Deprivation