PFKL antibody (Middle Region)

Catalog No. ABIN2782359

The Rabbit Polyclonal anti-PFKL antibody has been validated for WB. It is suitable to detect PFKL in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep and Zebrafish (Danio rerio). There is 1 publication available.

Quick Overview for PFKL antibody (Middle Region) (ABIN2782359)

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PFKL Antibody

Binding Specificity: Middle Region

Sequence: RTNVLGHLQQ GGAPTPFDRN YGTKLGVKAM LWLSEKLREV YRKGRVFANA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%, Sheep: 79%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against PFKL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PFKL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 568 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PFKL antibody (ABIN2782359)

Spellman, Ahmed, Dubach, Gardiner: "Expression of trisomic proteins in Down syndrome model systems." in: Gene, Vol. 512, Issue 2, pp. 219-25, (2012) (PubMed).

Target Details for PFKL

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Alternative Name: PFKL

Background: Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. PFK from muscle is a homotetramer of M subunit, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. PFKL represents the L subunit.
Alias Symbols: DKFZp686G1648, DKFZp686L2097, FLJ30173, FLJ40909, PFK-B
Protein Interaction Partner: KRTAP10-7, GTPBP3, PFKL, KRTAP5-9, FUS, STAU1, UBC, EGFR, RNF2, rev, ZC3HC1, PSMD9, FBXO6, YWHAQ, HDAC6, PRPF40A, TESK1, PFKM, MYD88, ITGA4, FN1, PCBP3, PRDX3, PFKP, APP, ATN1, RAD21, SNW1, TERF2, ATG101, TAF13, TAF10, DMWD, COPS6, KRTAP4-12,
Protein Size: 568

Molecular Weight: 64 kDa

Gene ID: 5211

NCBI Accession: NM_002626

UniProt: P17858

Pathways: Negative Regulation of Hormone Secretion, Warburg Effect