CCT8 antibody (C-Term)

Catalog No. ABIN2782379

The Rabbit Polyclonal anti-CCT8 antibody has been validated for WB. It is suitable to detect CCT8 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio) and Saccharomyces cerevisiae. There are 2+ publications available.

Quick Overview for CCT8 antibody (C-Term) (ABIN2782379)

Target: CCT8 (Chaperonin Containing TCP1, Subunit 8 (Theta) (CCT8))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCT8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CCT8 Antibody

Binding Specificity: C-Term

Sequence: DMLEAGILDT YLGKYWAIKL ATNAAVTVLR VDQIIMAKPA GGPKPPSGKK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against CCT8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CCT8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 548 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CCT8 antibody (ABIN2782379)

Spellman, Ahmed, Dubach, Gardiner: "Expression of trisomic proteins in Down syndrome model systems." in: Gene, Vol. 512, Issue 2, pp. 219-25, (2012) (PubMed).

Suzuki, Yoshitomo-Nakagawa, Maruyama, Suyama, Sugano: "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library." in: Gene, Vol. 200, Issue 1-2, pp. 149-56, (1997) (PubMed).

Target Details for CCT8

Target: CCT8 (Chaperonin Containing TCP1, Subunit 8 (Theta) (CCT8))

Alternative Name: CCT8

Background: As a molecular chaperone, CCT8 assists the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin.
Alias Symbols: Cctq, D21S246, KIAA0002, PRED71, C21orf112
Protein Interaction Partner: FUS, HUWE1, UBC, TP53, TUBG1, SUMO3, LGALS3BP, NEDD1, CDC20, SUMO1, NEDD8, MDM2, ASB13, CCT5, CCT2, CCT7, CCT6A, FBXO6, UBD, FBXW4, HDAC3, STK24, ILK, CDK9, CDK5, CDK2, PAN2, FN1, METTL21B, TP63, VCAM1, PEX14, NOS2, ITGA4, US3, FAM86B2, METTL20, METTL18,
Protein Size: 548

Molecular Weight: 59 kDa

Gene ID: 10694

NCBI Accession: NM_006585, NP_006576

UniProt: Q5RAP1