CENPA antibody (N-Term)

Catalog No. ABIN2782448

The Rabbit Polyclonal anti-CENPA antibody is suitable to detect CENPA in samples from Human, Rat and Mouse. It has been validated for WB and IHC.

Quick Overview for CENPA antibody (N-Term) (ABIN2782448)

Target: CENPA (Centromere Protein A (CENPA))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CENPA Antibody

Binding Specificity: N-Term

Sequence: MGPRRRSRKP EAPRRRSPSP TPTPGPSRRG PSLGASSHQH SRRRQGWLKE

Predicted Reactivity: Human: 100%, Mouse: 90%, Rat: 90%

Characteristics: This is a rabbit polyclonal antibody against CENPA. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CENPA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 140 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CENPA

Target: CENPA (Centromere Protein A (CENPA))

Alternative Name: CENPA

Background: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA is a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
Alias Symbols: CenH3, CENP-A
Protein Interaction Partner: HIST1H4A, HIST1H2AB, H3F3A, HIST1H2BB, DIAPH3, DIAPH1, NPM1, HIST1H1A, H2AFZ, H2AFX, CENPC, CENPB, HIST3H2BB, HIST4H4, CENPT, CENPU, CENPM, CENPH, CENPN, HJURP, SUPT16H, H2AFY, AURKB, HIST1H3E, HIST2H2AC, SSRP1, RUVBL1, XRCC5, PRKDC, HNRNPU, EIF4A1, DDX5,
Protein Size: 140

Molecular Weight: 16 kDa

Gene ID: 1058

NCBI Accession: NM_001809, NP_001800

UniProt: P49450

Pathways: Chromatin Binding, Maintenance of Protein Location