CENPA antibody (Middle Region)

Catalog No. ABIN502692

This anti-CENPA antibody is a Rabbit Polyclonal antibody detecting CENPA in WB. Suitable for Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Goat.

Quick Overview for CENPA antibody (Middle Region) (ABIN502692)

Target: CENPA (Centromere Protein A (CENPA))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CENPA Antibody

Binding Specificity: Middle Region

Sequence: ALQEAAEAFL VHLFEDAYLL TLHAGRVTLF PKDVQLARRI RGLEEGLG

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 77%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against CENPA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CENPA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 140 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CENPA

Target: CENPA (Centromere Protein A (CENPA))

Alternative Name: CENPA

Background: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA is a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Alias Symbols: CenH3, CENP-A
Protein Interaction Partner: HIST1H4A, HIST1H2AB, H3F3A, HIST1H2BB, DIAPH3, DIAPH1, NPM1, HIST1H1A, H2AFZ, H2AFX, CENPC, CENPB, HIST3H2BB, HIST4H4, CENPT, CENPU, CENPM, CENPH, CENPN, HJURP, SUPT16H, H2AFY, AURKB, HIST1H3E, HIST2H2AC, SSRP1, RUVBL1, XRCC5, PRKDC, HNRNPU, EIF4A1, DDX5,
Protein Size: 140

Molecular Weight: 16 kDa

Gene ID: 1058

NCBI Accession: NM_001809, NP_001800

UniProt: P49450

Pathways: Chromatin Binding, Maintenance of Protein Location