EDEM1 antibody (N-Term)

Catalog No. ABIN2782826

The Rabbit Polyclonal anti-EDEM1 antibody has been validated for WB. It is suitable to detect EDEM1 in samples from Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Dog and Saccharomyces cerevisiae.

Quick Overview for EDEM1 antibody (N-Term) (ABIN2782826)

Target: EDEM1 (ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Dog, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EDEM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EDEM1 Antibody

Binding Specificity: N-Term

Sequence: MAHAFPQDEL NPIHCRGRGP DRGDPSNLNI NDVLGNYSLT LVDALDTLAI

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 82%

Characteristics: This is a rabbit polyclonal antibody against EDEM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EDEM1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 657 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EDEM1

Target: EDEM1 (ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1))

Alternative Name: EDEM1

Background: EDEM1 belongs to the glycosyl hydrolase 47 family. It extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-dependent manner.
Alias Symbols: EDEM, KIAA0212
Protein Interaction Partner: HLA-B, SQSTM1, UBC, MMS19, SSR1, ASGR1, DERL1, DNAJC10, DERL2, SEC61B, SEL1L, RHO, CANX, RCOM_2159910, ASGR2, ELAVL1, BZW1,
Protein Size: 657

Molecular Weight: 74 kDa

Gene ID: 9695

NCBI Accession: NM_014674, NP_055489

UniProt: Q92611

Pathways: ER-Nucleus Signaling