SMC3 antibody (N-Term)

Catalog No. ABIN2783036

The Rabbit Polyclonal anti-SMC3 antibody has been validated for WB. It is suitable to detect SMC3 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio) and Guinea Pig.

Quick Overview for SMC3 antibody (N-Term) (ABIN2783036)

Target: SMC3 (Structural Maintenance of Chromosomes 3 (SMC3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMC3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMC3 Antibody

Binding Specificity: N-Term

Sequence: KWDKMRRALE YTIYNQELNE TRAKLDELSA KRETSGEKSR QLRDAQQDAR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against SMC3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SMC3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1217 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMC3

Target: SMC3 (Structural Maintenance of Chromosomes 3 (SMC3))

Alternative Name: SMC3

Background: SMC3 belongs to the SMC3 subfamily of SMC proteins. SMC3 occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation.
Alias Symbols: BAM, BMH, CDLS3, CSPG6, HCAP, SMC3L1
Protein Interaction Partner: SUMO2, UBC, RPA3, RPA2, RPA1, SUZ12, EED, RNF2, FBXO6, ANKRD28, HDAC11, HDAC8, HDAC9, SOX2, RAD21, CDCA5, PDS5A, WAPAL, MMS19, METTL21B, NOTCH1, SMC1A, SFPQ, HNRNPM, NSMCE2, UBD, COPS5, COPS6, CUL2, CUL3, NEDD8, CDK2, FBXO25, MECP2, NIPBL, CDK8, SUPT5H, P
Protein Size: 1217

Molecular Weight: 141 kDa

Gene ID: 9126

NCBI Accession: NM_005445, NP_005436

UniProt: Q9UQE7

Pathways: Stem Cell Maintenance