PHF5A antibody (Middle Region)

Catalog No. ABIN2783095

The Rabbit Polyclonal anti-PHF5A antibody has been validated for WB. It is suitable to detect PHF5A in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Goat.

Quick Overview for PHF5A antibody (Middle Region) (ABIN2783095)

Target: PHF5A (PHD Finger Protein 5A (PHF5A))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHF5A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PHF5A Antibody

Binding Specificity: Middle Region

Sequence: ICGGPGVSDA YYCKECTIQE KDRDGCPKIV NLGSSKTDLF YERKKYGFKK

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PHF5A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PHF5A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 110 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PHF5A

Target: PHF5A (PHD Finger Protein 5A (PHF5A))

Alternative Name: PHF5A

Background: This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes.
Alias Symbols: INI, MGC1346, SF3b14b, bK223H9.2, Rds3, SAP14b
Protein Interaction Partner: UBC, EZH2, BMI1, SUZ12, RNF2, SRPK3, FOXB1, PRPF31, SF3B5, RBM25, SF3B6, SF3B1, SF3B3, SF3B2, SF3B4, DDX23, SNRPB2, SNRPA1, SFPQ, S100A10, YBX1, NCL, HNRNPA1, DHX8, SF3A2, Sf3a1, SUMO2, RNU11, RNU12-2P,
Protein Size: 110

Molecular Weight: 12 kDa

Gene ID: 84844

NCBI Accession: NM_032758, NP_116147

UniProt: Q7RTV0