PLEKHO1 antibody (N-Term)

Catalog No. ABIN2783148

This Rabbit Polyclonal antibody specifically detects PLEKHO1 in WB. It exhibits reactivity toward Human, Rat, Cow and Pig.

Quick Overview for PLEKHO1 antibody (N-Term) (ABIN2783148)

Target: PLEKHO1 (Pleckstrin Homology Domain Containing, Family O Member 1 (PLEKHO1))

Reactivity: Human, Rat, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLEKHO1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PLEKHO1 Antibody

Binding Specificity: N-Term

Sequence: MMKKNNSAKR GPQDGNQQPA PPEKVGWVRK FCGKGIFREI WKNRYVVLKG

Predicted Reactivity: Cow: 86%, Human: 100%, Pig: 86%, Rat: 82%

Characteristics: This is a rabbit polyclonal antibody against PLEKHO1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PLEKHO1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 409 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PLEKHO1

Target: PLEKHO1 (Pleckstrin Homology Domain Containing, Family O Member 1 (PLEKHO1))

Alternative Name: PLEKHO1

Background: PLEKHO1 plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP).PLEKHO1 may function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). PLEKHO1 appears to target ATM to the plasma membrane. PLEKHO1 appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. PLEKHO1 is also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.
Alias Symbols: CKIP-1, OC120, RP11-458I7.3
Protein Interaction Partner: PLEKHO1, AKT3, AKT2, AKT1, UBC, GSK3B, TRAF6, Arpc1a, Stub1, Rps3a1, SMURF1, PSMC5, USP7, C10orf88, RPS20, IFI35, DNAJB1, FLNB, CSNK2A1, SMAD5, JUND, JUN, CASP3,
Protein Size: 409

Molecular Weight: 46 kDa

Gene ID: 51177

NCBI Accession: NM_016274, NP_057358

UniProt: Q53GL0