Nanog antibody (N-Term)

Catalog No. ABIN2783183

The Rabbit Polyclonal anti-Nanog antibody has been validated for WB. It is suitable to detect Nanog in samples from Human, Pig, Cow, Dog, Sheep, Goat and Horse.

Quick Overview for Nanog antibody (N-Term) (ABIN2783183)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human, Pig, Cow, Dog, Sheep, Goat, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nanog Antibody

Binding Specificity: N-Term

Sequence: ESSLTPVTCG PEENYPSLQM SSAEMPHAET VSPLPSSMDL LIQDSPDSST

Predicted Reactivity: Cow: 100%, Dog: 92%, Goat: 100%, Horse: 92%, Human: 100%, Pig: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against NANOG. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NANOG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 305 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background: NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
Alias Symbols: -
Protein Interaction Partner: DOT1L, UBC, ZNF281, RHOXF2, SALL4, MED12,
Protein Size: 305

Molecular Weight: 33 kDa

Gene ID: 79923

NCBI Accession: NM_024865, NP_079141

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance