EIF4A1 antibody (Middle Region)

Catalog No. ABIN2783213

The Rabbit Polyclonal anti-EIF4A1 antibody has been validated for WB. It is suitable to detect EIF4A1 in samples from Human, Rat, Mouse, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Dog, Saccharomyces cerevisiae and Sheep. There is 1 publication available.

Quick Overview for EIF4A1 antibody (Middle Region) (ABIN2783213)

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Reactivity: Human, Rat, Mouse, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Dog, Saccharomyces cerevisiae, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4A1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF4A1 Antibody

Binding Specificity: Middle Region

Sequence: TMPSDVLEVT KKFMRDPIRI LVKKEELTLE GIRQFYINVE REEWKLDTLC

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%, Yeast: 92%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF4A1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EIF4A1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 406 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EIF4A1 antibody (ABIN2783213)

Tamarkin-Ben-Harush, Vasseur, Debart, Ulitsky, Dikstein: "Cap-proximal nucleotides via differential eIF4E binding and alternative promoter usage mediate translational response to energy stress." in: eLife, Vol. 6, (2017) (PubMed).

Target Details for EIF4A1

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Alternative Name: EIF4A1

Background: EIF4A1 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Alias Symbols: DDX2A, EIF-4A, EIF4A, eIF4A-I, eIF-4A-I
Protein Interaction Partner: HUWE1, UBC, FUS, SUMO2, SUMO3, STAU1, MDM2, SH3RF2, TRAP1, FBXO6, ADRB2, CD81, NPM1, VCAM1, ITGA4, FN1, ESR1, LIG4, RPLP0P6, TMEM43, NDUFB11, ZBTB43, GPRC5A, EIF4G3, EIF4H, VDAC3, UQCRFS1, SDHA, RPS26, RPL19, RPL8, RPSA, ILF2, HSP90AB1, HSPA5, EIF4G2, EIF
Protein Size: 406

Molecular Weight: 46 kDa

Gene ID: 1973

NCBI Accession: NM_001416, NP_001407

UniProt: P60842