eEF1A1 antibody (C-Term)

Catalog No. ABIN2783254

This Rabbit Polyclonal antibody specifically detects eEF1A1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Sheep, Rabbit, Horse, Guinea Pig and Goatand has been mentioned in 1 publication.

Quick Overview for eEF1A1 antibody (C-Term) (ABIN2783254)

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep, Rabbit, Horse, Guinea Pig, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eEF1A1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-eEF1A1 Antibody

Binding Specificity: C-Term

Sequence: IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against EEF1A1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EEF1A1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 462 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-eEF1A1 antibody (ABIN2783254)

Yan, Zhang, Chen, Gutmann, Xiong, Xiao, Huang: "Reduced T-cadherin expression and promoter methylation are associated with the development and progression of hepatocellular carcinoma." in: International journal of oncology, Vol. 32, Issue 5, pp. 1057-63, (2008) (PubMed).

Target Details for eEF1A1

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Alternative Name: EEF1A1

Background: EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66 % of patients with Felty syndrome.This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66 % of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CCS-3, CCS3, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, LENG7, MGC102687, MGC131894, MGC16224, PTI1, eEF1A-1, EE1A1
Protein Interaction Partner: GPX7, FUS, UBC, ISG15, SUMO3, CEP250, TUBGCP2, TUBG1, TP53, AURKA, SUMO2, STAU1, ALPL, LGR4, MDM2, RPA3, RPA2, RPA1, SHFM1, rev, PPP1CB, PFKP, GAPDH, PARK2, TRAP1, FBXO6, EGFR, ADRB2, RAD52, NPM1, CD81, BBS4, BBS2, BBS1, AICDA, STAT6, RBBP8, FN1, CDC25A,
Protein Size: 462

Molecular Weight: 50 kDa

Gene ID: 1915

NCBI Accession: NM_001402, NP_001393

UniProt: P68104