CRYAB antibody (C-Term)

Catalog No. ABIN2783274

The Rabbit Polyclonal anti-CRYAB antibody has been validated for WB. It is suitable to detect CRYAB in samples from Human, Rat, Mouse, Cow, Sheep, Dog, Guinea Pig, Rabbit and Horse.

Quick Overview for CRYAB antibody (C-Term) (ABIN2783274)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reactivity: Human, Rat, Mouse, Cow, Sheep, Dog, Guinea Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRYAB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CRYAB Antibody

Binding Specificity: C-Term

Sequence: KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVT

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%, Sheep: 92%

Characteristics: This is a rabbit polyclonal antibody against CRYAB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CRYAB

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 175 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: CRYAB

Background: Alpha crystallins are composed of: alpha-A and alpha-B, for acidic and basic, respectively. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone, instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B are differentially expressed, alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases, a missense mutation cosegregated in a family with a desmin-related myopathy.
Alias Symbols: CRYA2, CTPP2, HSPB5
Protein Interaction Partner: CRYAB, CRYAA, KRTAP19-5, KRTAP8-1, TRAPPC6A, MRPL11, TNPO2, ACOT7, HSPB1, CASP7, CASP3, CASP2, PIDD1, DDX20, CRADD, B2M, APOC2, XIAP, CASP12, SOD1, HSPB2, HBA1, CASP9, CASP8, CASP6, ASB5, SMAD4, TRIM33, UBC, CCL22, CTNNB1, CRYGD, CDH1, SNCA, GFAP, FGF2, F
Protein Size: 175

Molecular Weight: 20 kDa

Gene ID: 1410

NCBI Accession: NM_001885

UniProt: P02511