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CRYAB antibody (FITC)

CRYAB Reactivity: Human WB, IHC, IF, ICC Host: Rabbit Polyclonal FITC
Catalog No. ABIN2486810
  • Target See all CRYAB Antibodies
    CRYAB (Crystallin, alpha B (CRYAB))
    Reactivity
    • 130
    • 97
    • 84
    • 15
    • 9
    • 8
    • 6
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 1
    Human
    Host
    • 158
    • 33
    • 2
    • 1
    Rabbit
    Clonality
    • 151
    • 43
    Polyclonal
    Conjugate
    • 77
    • 11
    • 10
    • 9
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    This CRYAB antibody is conjugated to FITC
    Application
    • 139
    • 74
    • 66
    • 66
    • 56
    • 35
    • 33
    • 32
    • 17
    • 15
    • 11
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Detects ~22 kDa. Does not cross-react with αA-crystallin.
    Cross-Reactivity
    Chicken, Cow, Human, Mouse, Rat
    Purification
    Affinity Purified
    Immunogen
    Synthetic peptide corresponding to human alpha B crystallin conjugated to KLH
    Top Product
    Discover our top product CRYAB Primary Antibody
  • Application Notes
    • WB (1:5000)
    • ICC/IF (1:120)
    • optimal dilutions for assays should be determined by the user.
    Comment

    A 1:5000 dilution of ABIN2486810 was sufficient for detection of alpha B crystallin in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    CRYAB (Crystallin, alpha B (CRYAB))
    Alternative Name
    Alpha B Crystallin (CRYAB Products)
    Synonyms
    CMD1II antibody, CRYA2 antibody, CTPP2 antibody, CTRCT16 antibody, HSPB5 antibody, MFM2 antibody, Crya-2 antibody, Crya2 antibody, HspB5 antibody, AACRYA antibody, CRYAB antibody, cryab antibody, cryab2 antibody, wu:fe37f08 antibody, zgc:91937 antibody, crystallin alpha B antibody, crystallin, alpha B antibody, hypothetical protein antibody, crystallin, alpha B, a antibody, crystallin, alpha B, b antibody, CRYAB antibody, Cryab antibody, ZK1128.7 antibody, cryaba antibody, cryabb antibody
    Background
    The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).
    Gene ID
    1410
    NCBI Accession
    NP_001876
    UniProt
    P02511
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