ENO3 antibody (N-Term)

Catalog No. ABIN2783278

This anti-ENO3 antibody is a Rabbit Polyclonal antibody detecting ENO3 in WB and IHC. Suitable for Human, Mouse, Rat, Rabbit, Cow, Dog, Goat, Guinea Pig, Horse, Zebrafish (Danio rerio) and Saccharomyces cerevisiae. This Primary Antibody has been cited in 2+ publications.

Quick Overview for ENO3 antibody (N-Term) (ABIN2783278)

Target: ENO3 (Enolase 3 (Beta, Muscle) (ENO3))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Goat, Guinea Pig, Horse, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ENO3 Antibody

Binding Specificity: N-Term

Sequence: MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against ENO3. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ENO3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 434 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ENO3 antibody (ABIN2783278)

Gergs, Jahn, Schulz, Großmann, Rueckschloss, Demus, Buchwalow, Neumann: "Protein Phosphatase 2A Improves Cardiac Functional Response to Ischemia and Sepsis." in: International journal of molecular sciences, Vol. 23, Issue 9, (2022) (PubMed).

Chaika, Yu, Purohit, Mehla, Lazenby, DiMaio, Anderson, Yeh, Johnson, Hollingsworth, Singh: "Differential expression of metabolic genes in tumor and stromal components of primary and metastatic loci in pancreatic adenocarcinoma." in: PLoS ONE, Vol. 7, Issue 3, pp. e32996, (2012) (PubMed).

Target Details for ENO3

Target: ENO3 (Enolase 3 (Beta, Muscle) (ENO3))

Alternative Name: ENO3

Background: ENO3 is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR.
Alias Symbols: MSE, GSD13
Protein Interaction Partner: UBC, IQCB1, DAK, PKM, GPI, ENO1, EEF1A1, APP, SUMO1, PNKD, TRIM63,
Protein Size: 434

Molecular Weight: 47 kDa

Gene ID: 2027

NCBI Accession: NM_001976, NP_001967

UniProt: P13929