PFN1 antibody (N-Term)

Catalog No. ABIN2783306

This Rabbit Polyclonal antibody specifically detects PFN1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig, Horse and Zebrafish (Danio rerio)and has been mentioned in 1 publication.

Quick Overview for PFN1 antibody (N-Term) (ABIN2783306)

Target: PFN1 (Profilin 1 (PFN1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PFN1 Antibody

Binding Specificity: N-Term

Sequence: AGWNAYIDNL MADGTCQDAA IVGYKDSPSV WAAVPGKTFV NITPAEVGVL

Predicted Reactivity: Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against PFN1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PFN1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 140 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PFN1 antibody (ABIN2783306)

Witt, Büttner, Jannasch, Matschke, Waldow: "Reversal of myofibroblastic activation by polyunsaturated fatty acids in valvular interstitial cells from aortic valves. Role of RhoA/G-actin/MRTF signalling." in: Journal of molecular and cellular cardiology, Vol. 74, pp. 127-38, (2014) (PubMed).

Target Details for PFN1

Target: PFN1 (Profilin 1 (PFN1))

Alternative Name: PFN1

Background: PFN1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome.The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: -
Protein Interaction Partner: UBC, FUS, STUB1, SUMO1, NEDD8, MDM2, ASB12, ASB2, ASB1, YWHAQ, SRPK1, ESR1, RAD52, RAD51, ACTB, VCAM1, ITGA4, FN1, ATF2, LIG4, SET, ACAA2, LOC440434, MRPL53, SRPRB, CAND1, CUL3, CDK2, ISG15, ELAVL1, AI837181, Akap12, Csnk1e, Mapk13, UCHL5, WIPF2, LRIF1, M
Protein Size: 140

Molecular Weight: 15 kDa

Gene ID: 5216

NCBI Accession: NM_005022, NP_005013

UniProt: P07737

Pathways: Regulation of Actin Filament Polymerization, Tube Formation, Maintenance of Protein Location