MDH1 antibody (Middle Region)

Catalog No. ABIN2783317

This Rabbit Polyclonal antibody specifically detects MDH1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Horse, Guinea Pig, Dog, Zebrafish (Danio rerio) and Goat and has been mentioned in 2+ publications.

Quick Overview for MDH1 antibody (Middle Region) (ABIN2783317)

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Horse, Guinea Pig, Dog, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MDH1 Antibody

Binding Specificity: Middle Region

Sequence: NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV

Predicted Reactivity: Cow: 100%, Dog: 93%, Goat: 92%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against MDH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MDH1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 334 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MDH1 antibody (ABIN2783317)

Nussbaumer, Asara, Teplytska, Murphy, Logan, Turck, Filiou: "Selective Mitochondrial Targeting Exerts Anxiolytic Effects In Vivo." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 41, Issue 7, pp. 1751-8, (2016) (PubMed).

Povlsen, Løfgren, Dalgas, Birkler, Johannsen, Støttrup, Bøtker: "Protection against myocardial ischemia-reperfusion injury at onset of type 2 diabetes in Zucker diabetic fatty rats is associated with altered glucose oxidation." in: PLoS ONE, Vol. 8, Issue 5, pp. e64093, (2013) (PubMed).

Target Details for MDH1

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Alternative Name: MDH1

Background: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MDH-s, MDHA, MGC:1375, MOR2
Protein Interaction Partner: ISG15, UBC, NEDD8, PARK2, HSP90AB1, HSP90AA1, FUS, IQCB1, SDHA, PGM1, LDHA, ESD, Htt, PRDX5, PRDX6, SLC9A3R1, AKT1, TERF2IP, TERF1, TP53, EGFR, MPP3, MDH1, GPD1,
Protein Size: 334

Molecular Weight: 36 kDa

Gene ID: 4190

NCBI Accession: NM_005917, NP_005908

UniProt: P40925