NEK2 antibody (N-Term)

Catalog No. ABIN2783428

This Rabbit Polyclonal antibody specifically detects NEK2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig, Horse, Dog, Rabbit and Zebrafish (Danio rerio).

Quick Overview for NEK2 antibody (N-Term) (ABIN2783428)

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Dog, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NEK2 Antibody

Binding Specificity: N-Term

Sequence: RCQKIRRKSD GKILVWKELD YGSMTEAEKQ MLVSEVNLLR ELKHPNIVRY

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against NEK2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human NEK2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 445 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NEK2

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Alternative Name: NEK2

Background: This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene.
Alias Symbols: HsPK21, NEK2A, NLK1
Protein Interaction Partner: ANAPC4, UBC, NEK2, CEP250, CTNNB1, PPP1CC, VCP, CDH1, CDC20, CHEK1, CSN2, CDC27, MYC, PPP1CA, CCDC85A, SPTBN4, GIT1, EDRF1, TCF25, IMMT, NDC80, IFT140, PDE4DIP, TPM3, MCC, EIF4A2, DCTN1, CHGA, Gm9174, Cdc16, Cdc23, NEK11, MAD1L1, PPP1R2, MAPK1, CLEC4C,
Protein Size: 445

Molecular Weight: 52 kDa

Gene ID: 4751

NCBI Accession: NM_002497, NP_002488

UniProt: P51955

Pathways: M Phase