POLR3F antibody (N-Term)

Catalog No. ABIN2783471

The Rabbit Polyclonal anti-POLR3F antibody is suitable to detect POLR3F in samples from Human, Mouse, Rat, Horse, Rabbit, Cow, Guinea Pig, Dog and Pig. It has been validated for WB.

Quick Overview for POLR3F antibody (N-Term) (ABIN2783471)

Target: POLR3F (Polymerase (RNA) III (DNA Directed) Polypeptide F, 39 KDa (POLR3F))

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Cow, Guinea Pig, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3F antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR3F Antibody

Binding Specificity: N-Term

Sequence: MAEVKVKVQP PDADPVEIEN RIIELCHQFP HGITDQVIQN EMPHIEAQQR

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against POLR3F. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POLR3F

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 316 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLR3F

Target: POLR3F (Polymerase (RNA) III (DNA Directed) Polypeptide F, 39 KDa (POLR3F))

Alternative Name: POLR3F

Background: POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.
Alias Symbols: MGC13517, RPC39, RPC6
Protein Interaction Partner: SUV39H2, PRPF4B, UBE2G2, UBC, POLR3G, POLR2E, POLR2B, RBM28, RSL1D1, RRP12, POLR3C, ELAVL1, LRIF1, WDR89, GTF3C4, BDP1, BRF1, C14orf1, STC2, KAT5, POLR3D, TBP,
Protein Size: 316

Molecular Weight: 36 kDa

Gene ID: 10621

NCBI Accession: NM_006466, NP_006457

UniProt: Q9H1D9