POLR3A antibody (Middle Region)

Catalog No. ABIN2783481

The Rabbit Polyclonal anti-POLR3A antibody is suitable to detect POLR3A in samples from Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for POLR3A antibody (Middle Region) (ABIN2783481)

Target: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR3A Antibody

Binding Specificity: Middle Region

Sequence: AYFGQKDSVC GVSECIIMGI PMNIGTGLFK LLHKADRDPN PPKRPLIFDT

Predicted Reactivity: Cow: 79%, Dog: 86%, Guinea Pig: 86%, Horse: 79%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 86%, Yeast: 85%

Characteristics: This is a rabbit polyclonal antibody against POLR3A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POLR3A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1390 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLR3A

Target: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Alternative Name: POLR3A

Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
Alias Symbols: RPC1, RPC155, hRPC155, ADDH, HLD7
Protein Interaction Partner: SUMO2, UBC, ASB13, NEDD4, POLR3H, VPS18, POLR3B, POLR1D, CRCP, POLR1A, POLR3C, POLR1C, SMARCA5, POLR2H, POLR2E, POLR3D, IKBKG, PCBD1, EWSR1, RPAP2, RPAP3, RPAP1, GPN1, RAE1, POLR3E, PKP2, GTF2B,
Protein Size: 1390

Molecular Weight: 156 kDa

Gene ID: 11128

NCBI Accession: NM_007055, NP_008986

UniProt: O14802