POLK antibody (N-Term)

Catalog No. ABIN2783505

The Rabbit Polyclonal anti-POLK antibody has been validated for WB. It is suitable to detect POLK in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Pig.

Quick Overview for POLK antibody (N-Term) (ABIN2783505)

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLK Antibody

Binding Specificity: N-Term

Sequence: KINKIIMEAT KGSRFYGNEL KKEKQVNQRI ENMMQQKAQI TSQQLRKAQL

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against POLK. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POLK

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 870 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLK

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Alternative Name: POLK

Background: POLK belongs to the DNA polymerase type-Y family. It contains 2 Rad18-type zinc fingers and 1 umuC domain. POLK is a DNA polymerase specifically involved in DNA repair. It plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. It lacks 3'-5' proofreading exonuclease activity. POLK forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
Alias Symbols: DINB1, DINP, POLQ
Protein Interaction Partner: XPO7, MSH2, MCM6, MCM4, MSH6, TRAIP, UBC, REV1, Shprh, RAD18, PCNA, XRCC1, RPA1, POLD1,
Protein Size: 870

Molecular Weight: 99 kDa

Gene ID: 51426

NCBI Accession: NM_016218, NP_057302

UniProt: Q9UBT6

Pathways: DNA Damage Repair