POLR1B antibody (Middle Region)

Catalog No. ABIN2783529

This Rabbit Polyclonal antibody specifically detects POLR1B in WB. It exhibits reactivity toward Human, Mouse, Rat, Pig, Rabbit, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig and Horse.

Quick Overview for POLR1B antibody (Middle Region) (ABIN2783529)

Target: POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR1B Antibody

Binding Specificity: Middle Region

Sequence: SDKFQVRTTG ARDRVTNQPI GGRNVQGGIR FGEMERDALL AHGTSFLLHD

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 100%, Rat: 93%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against POLR1B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POLR1B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1135 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLR1B

Target: POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))

Alternative Name: POLR1B

Background: Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).
Alias Symbols: FLJ10816, FLJ21921, MGC131780, RPA135, Rpo1-2, RPA2
Protein Interaction Partner: UBC, RPA3, RPA2, RPA1, RRN3, HDAC6, GIT2, MED20, NMI, LDB1, ZNF207, RPL37, CCND1, AKT2, ACTA1, ERBB2, ACTB, TSR1, POLR1D, POLR1A, CD3EAP, NOP56, POLR1C, DHX8, CAND1, ARRB2, LZTR1, TAF1D, USP11, POLR1E, EIF3L, NFKBIB, POLR1B, ESR1, RIBIN,
Protein Size: 1135

Molecular Weight: 128 kDa

Gene ID: 84172

NCBI Accession: NM_019014, NP_061887

UniProt: Q9H9Y6