CHST14 antibody (Middle Region)

Catalog No. ABIN2783597

The Rabbit Polyclonal anti-CHST14 antibody has been validated for WB. It is suitable to detect CHST14 in samples from Human, Mouse, Rat, Cow, Dog, Pig and Guinea Pig. There is 1 publication available.

Quick Overview for CHST14 antibody (Middle Region) (ABIN2783597)

Target: CHST14 (Carbohydrate (N-Acetylgalactosamine 4-0) Sulfotransferase 14 (CHST14))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHST14 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHST14 Antibody

Binding Specificity: Middle Region

Sequence: REYQQRYGAE IVRRYRAGAG PSPAGDDVTF PEFLRYLVDE DPERMNEHWM

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CHST14. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CHST14

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 338 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CHST14 antibody (ABIN2783597)

Janecke, Li, Boehm, Krabichler, Rohrbach, Müller, Fuchs, Golas, Katagiri, Ziegler, Gahl, Wilnai, Zoppi, Geller, Giunta, Slavotinek, Steinmann: "The phenotype of the musculocontractural type of Ehlers-Danlos syndrome due to CHST14 mutations." in: American journal of medical genetics. Part A, Vol. 170A, Issue 1, pp. 103-15, (2016) (PubMed).

Target Details for CHST14

Target: CHST14 (Carbohydrate (N-Acetylgalactosamine 4-0) Sulfotransferase 14 (CHST14))

Alternative Name: CHST14

Background: CHST14 belongs to the sulfotransferase 2 family. CHST14 catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. It transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. It transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. CHST14 has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA.
Alias Symbols: D4ST-1, D4ST1, HD4ST, HNK1ST, ATCS
Protein Interaction Partner: UBD,
Protein Size: 338

Molecular Weight: 35 kDa

Gene ID: 113189

UniProt: Q8NCH0

Pathways: Glycosaminoglycan Metabolic Process