MAP4K4 antibody (N-Term)

Catalog No. ABIN2783612

This Rabbit Polyclonal antibody specifically detects MAP4K4 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow and Zebrafish (Danio rerio).

Quick Overview for MAP4K4 antibody (N-Term) (ABIN2783612)

Target: MAP4K4 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MAP4K4 Antibody

Binding Specificity: N-Term

Sequence: PFIRDQPNER QVRIQLKDHI DRTRKKRGEK DETEYEYSGS EEEEEEVPEQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MAP4K4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MAP4K4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1166 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MAP4K4

Target: MAP4K4 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4))

Alternative Name: MAP4K4

Background: MAP4K4 is the serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. MAP4K4 appears to act upstream of the JUN N-terminal pathway.The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Alias Symbols: FLH21957, FLJ10410, FLJ20373, FLJ90111, HGK, KIAA0687, NIK, MEKKK4
Protein Interaction Partner: UBC, MOB1B, NME7, PPP1CC, HSP90AA1, PAXIP1, GRK5, TRAPPC9, PPP1CA, KIF26B, HIST2H2BE, SLC9A1, PRKCE, KIF5A, ZRANB1, DOK1, MAP3K7, NCK1, GBP3, RAP2A, RASA1, ITGB1, MAP3K1, BRCA1, ATL1,
Protein Size: 1239

Molecular Weight: 142 kDa

Gene ID: 9448

NCBI Accession: NM_004834, NP_004825

UniProt: O95819