HELT antibody (Middle Region)

Catalog No. ABIN2784174

This Rabbit Polyclonal antibody specifically detects HELT in WB. It exhibits reactivity toward Mouse, Human, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Dog.

Quick Overview for HELT antibody (Middle Region) (ABIN2784174)

Target: HELT (Helt BHLH Transcription Factor (HELT))

Reactivity: Mouse, Human, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HELT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HELT Antibody

Binding Specificity: Middle Region

Sequence: EMTVQYLRAL HSADFPRGRE KAELLAEFAN YFHYGYHECM KNLVHYLTTV

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against HELT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HELT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 327 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HELT

Target: HELT (Helt BHLH Transcription Factor (HELT))

Alternative Name: HELT

Background: HELT belongs to the HEY family. It contains 1 basic helix-loop-helix (bHLH) domain and 1orange domain. HELT is a transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGCG-3'.
Alias Symbols: HCM1228, HESL, Mgn, bHLHb44
Protein Interaction Partner: TADA2A,
Protein Size: 327

Molecular Weight: 36 kDa

Gene ID: 391723

NCBI Accession: NM_001029887, NP_001025058

UniProt: A6NFD8

Pathways: Feeding Behaviour