SIN3B antibody (Middle Region)

Catalog No. ABIN2784243

This Rabbit Polyclonal antibody specifically detects SIN3B in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Pig, Guinea Pig and Zebrafish (Danio rerio)and has been mentioned in 1 publication.

Quick Overview for SIN3B antibody (Middle Region) (ABIN2784243)

Target: SIN3B (SIN3 Transcription Regulator Homolog B (SIN3B))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIN3B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SIN3B Antibody

Binding Specificity: Middle Region

Sequence: LVSDDVCLKV VELYLNEKKR GAAGGNLSSR CVRAARETSY QWKAERCMAD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 100%, Rat: 79%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against SIN3B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SIN3B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1162 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SIN3B antibody (ABIN2784243)

Dhanda, Lindberg, Olsson: "The human SIN3B corepressor forms a nucleolar complex with leukemia-associated ETO homologues." in: BMC molecular biology, Vol. 9, pp. 8, (2008) (PubMed).

Target Details for SIN3B

Target: SIN3B (SIN3 Transcription Regulator Homolog B (SIN3B))

Alternative Name: SIN3B

Background: SIN3B acts as a transcriptional repressor. It interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also, it interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. The gene is also able to form a complex with FOXK1 which represses transcription
Alias Symbols: KIAA0700
Protein Interaction Partner: SOX2, HDAC1, HDAC2, UBC, ESR1, AR, TFAP4, TP53, SUMO2, KLF16, MORF4L1, MORF4L2, MRGBP, KDM5A, CIITA, NR1D2, COPS2, SMN1, PBX1, REST, ZBTB4, ARID4A, MYT1, MXI1, SAP25, SMARCE1, SMARCB1, SMARCA4, SMARCA2, CBFA2T3, RNF220, ZBTB16, SETDB1, HDAC9, IKZF4, IKZF1
Protein Size: 1162

Molecular Weight: 133 kDa

Gene ID: 23309

NCBI Accession: NM_015260, NP_056075

UniProt: O75182