SPRY4 antibody (Middle Region)

Catalog No. ABIN2784290

The Rabbit Polyclonal anti-SPRY4 antibody (ABIN2784290) specifically detects SPRY4 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig, Horse and Rabbit samples.

Quick Overview for SPRY4 antibody (Middle Region) (ABIN2784290)

Target: SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRY4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPRY4 Antibody

Binding Specificity: Middle Region

Sequence: LCYLPATGCV KLAQRGYDRL RRPGCRCKHT NSVICKAASG DAKTSRPDKP

Predicted Reactivity: Cow: 86%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 86%, Rabbit: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against SPRY4. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SPRY4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 322 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SPRY4

Target: SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))

Alternative Name: SPRY4

Background: SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS (see HRAS, MIM 190020) activation and impairs the formation of active GTP-RAS (Leeksma et al., 2002 [PubMed 12027893]).
Alias Symbols: -
Protein Interaction Partner: GRB2, SPRY2, SPRY4, TESK1, RAF1, CBL,
Protein Size: 322

Molecular Weight: 35 kDa

Gene ID: 81848

NCBI Accession: NM_030964, NP_112226

UniProt: Q9C004