DUSP8 antibody (Middle Region)

Catalog No. ABIN2784750

This Rabbit Polyclonal antibody specifically detects DUSP8 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog and Guinea Pig.

Quick Overview for DUSP8 antibody (Middle Region) (ABIN2784750)

Target: DUSP8 (Dual Specificity Phosphatase 8 (DUSP8))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DUSP8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DUSP8 Antibody

Binding Specificity: Middle Region

Sequence: PAPPTPPATS ALQQGLRGLH LSSDRLQDTN RLKRSFSLDI KSAYAPSRRP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against DUSP8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DUSP8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 625 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DUSP8

Target: DUSP8 (Dual Specificity Phosphatase 8 (DUSP8))

Alternative Name: DUSP8

Background: DUSP8 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP8inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin.The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2.
Alias Symbols: C11orf81, FLJ42476, FLJ42958, HB5, HVH-5, HVH8
Protein Interaction Partner: MAPK9, MAPK8, CDC25A,
Protein Size: 625

Molecular Weight: 66 kDa

Gene ID: 1850

NCBI Accession: NM_004420, NP_004411

UniProt: Q13202