Protein Tob1 (TOB1) (Middle Region) antibody

Catalog No. ABIN2784777

The Rabbit Polyclonal anti- antibody has been validated for WB. It is suitable to detect in samples from Human, Mouse, Rat, Cow, Dog, Horse, Pig and Saccharomyces cerevisiae.

Quick Overview for Protein Tob1 (TOB1) (Middle Region) antibody (ABIN2784777)

Target: Protein Tob1 (TOB1)

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: Middle Region

Sequence: DLLKQKAISS SMHSLYGLGL GSQQQPQQQQ QPAQPPPPPP PPQQQQQQKT

Predicted Reactivity: Cow: 93%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 82%, Pig: 93%, Rat: 92%, Yeast: 100%

Characteristics: This is a rabbit polyclonal antibody against TOB1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TOB1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 345 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: Protein Tob1 (TOB1)

Alternative Name: TOB1

Background: This gene encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.
Alias Symbols: APRO6, MGC104792, MGC34446, PIG49, TOB, TROB, TROB1
Protein Interaction Partner: CORO7, APP, DTL, CDC7, PABPC1, CHAF1A, PAN2, UBR5, ELAVL1, SMAD3, CTNNB1, UBC, SKP2, SCMH1, MARCKS, SETDB1, CNOT7, SMAD9, SMAD5, SMAD6, SMAD1, SMAD2, SMAD4, MAPK1, ERBB2, RPS6KA1, MAPK9,
Protein Size: 345

Molecular Weight: 38 kDa

Gene ID: 10140

NCBI Accession: NM_005749, NP_005740

UniProt: P50616