NUP50 antibody (C-Term)

Catalog No. ABIN2784862

This Rabbit Polyclonal antibody specifically detects NUP50 in WB and IF. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Pig and Saccharomyces cerevisiae.

Quick Overview for NUP50 antibody (C-Term) (ABIN2784862)

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUP50 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-NUP50 Antibody

Binding Specificity: C-Term

Sequence: TTQSKPVSSP FPTKPLEGQA EGDSGECKGG DEEENDEPPK VVVTEVKEED

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 85%, Horse: 100%, Human: 100%, Mouse: 91%, Pig: 100%, Rabbit: 100%, Rat: 92%, Yeast: 80%

Characteristics: This is a rabbit polyclonal antibody against NUP50. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NUP50

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 468 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NUP50

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Alternative Name: NUP50

Background: The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import.The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: MGC39961, NPAP60, NPAP60L
Protein Interaction Partner: KPNA5, KPNA4, KPNA3, KPNA2, KPNA1, KPNA6, UBC, KIAA1033, SART3, TTI1, TSC22D1, IKBKAP, KIF5B, EPB41L1, ACACA, SRPK2, LMNA, HDAC6, NPM1, UBAP2, TOR1AIP1, NUP153, APP, CUL3, Mki67, Kifc5b, SLX4, KPNB1, XPO1, RAN, CDKN1B, IPO13,
Protein Size: 468

Molecular Weight: 50 kDa

Gene ID: 10762

NCBI Accession: NM_007172, NP_009103

UniProt: Q9UKX7

Pathways: Tube Formation