EGLN3 antibody (C-Term)

Catalog No. ABIN2784911

This Rabbit Polyclonal antibody specifically detects EGLN3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Sheep and Zebrafish (Danio rerio).

Quick Overview for EGLN3 antibody (C-Term) (ABIN2784911)

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGLN3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EGLN3 Antibody

Binding Specificity: C-Term

Sequence: FWSDRRNPHE VQPSYATRYA MTVWYFDAEE RAEAKKKFRN LTRKTESALT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 77%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against EGLN3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EGLN3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 239 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EGLN3

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Alternative Name: EGLN3

Background: EGLN3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. EGLN3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. EGLN3 promotes cell death through a caspase-dependent mechanism.
Alias Symbols: FLJ21620, HIFPH3, MGC125998, MGC125999, PHD3
Protein Interaction Partner: FOXJ2, SERTAD1, NCAPH2, EFHC2, ABI2, SIAH2, REL, HIF1A, TTC23L, SQSTM1, HEXIM1, IKBKG, IKBKB, CHUK, MAPK7, MAPK6, MAPK1, PKM, EPAS1, UBD, MAGEA11, UBC, ATF4, WTIP, AJUBA, LIMD1, SPRY2, ADRB2, PRPF19, Myog, EGLN3, EGLN2, EGLN1, OS9, HIF3A, WDR83,
Protein Size: 239

Molecular Weight: 27 kDa

Gene ID: 112399

NCBI Accession: NM_022073, NP_071356

UniProt: Q9H6Z9

Pathways: Positive Regulation of Endopeptidase Activity