UBE2F antibody (Middle Region)

Catalog No. ABIN2784962

The Rabbit Polyclonal anti-UBE2F antibody has been validated for WB. It is suitable to detect UBE2F in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Saccharomyces cerevisiae.

Quick Overview for UBE2F antibody (Middle Region) (ABIN2784962)

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2F antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE2F Antibody

Binding Specificity: Middle Region

Sequence: TLKDVVWGLN SLFTDLLNFD DPLNIEAAEH HLRDKEDFRN KVDDYIKRYA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 79%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against UBE2F. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE2F

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 185 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Alternative Name: UBE2F

Background: UBE2F belongs to the ubiquitin-conjugating enzyme family. UBE2F accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins.
Alias Symbols: MGC18120, NCE2
Protein Interaction Partner: CUL1, UBC, NEDD8, DCUN1D3, DCUN1D2, DCUN1D1, RNF7, CUL5, S100A11, vif, ELAVL1, RNF34,
Protein Size: 185

Molecular Weight: 21 kDa

Gene ID: 140739

NCBI Accession: NM_080678, NP_542409

UniProt: Q969M7