MLKL antibody (N-Term)

Catalog No. ABIN2785223

Product Details anti-MLKL Antibody

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Binding Specificity: N-Term

Reactivity: Human, Rat, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLKL antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DVWKELSLLL QVEQRMPVSP ISQGASWAQE DQQDADEDRR AFQMLRRDNE

Predicted Reactivity: Cow: 85%, Horse: 91%, Human: 100%, Rat: 90%

Characteristics: This is a rabbit polyclonal antibody against MLKL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MLKL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 471 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MLKL antibody (ABIN2785223)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for MLKL

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Alternative Name: MLKL (MLKL Products)

Background: The specific function of this protein remains unknown.
Alias Symbols: FLJ34389
Protein Interaction Partner: UBC, THOC3, RAD18, ANAPC11, NDUFB7, CDK1, PITPNA,
Protein Size: 471

Molecular Weight: 54 kDa

Gene ID: 197259

NCBI Accession: NM_152649, NP_689862

UniProt: Q8NB16