Importin 5 antibody (N-Term)

Catalog No. ABIN2785469

The Rabbit Polyclonal anti-Importin 5 antibody has been validated for WB. It is suitable to detect Importin 5 in samples from Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for Importin 5 antibody (N-Term) (ABIN2785469)

Target: Importin 5 (IPO5)

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Importin 5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Importin 5 Antibody

Binding Specificity: N-Term

Sequence: GNNQWPEGLK FLFDSVSSQN VGLREAALHI FWNFPGIFGN QQQHYLDVIK

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against RANBP5. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RANBP5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1115 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Importin 5

Target: Importin 5 (IPO5)

Alternative Name: RANBP5

Background: Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.
Alias Symbols: DKFZp686O1576, FLJ43041, IMB3, KPNB3, MGC2068, RANBP5, imp5
Protein Interaction Partner: HUWE1, UBC, STAU1, ASB15, HK2, HEXB, ATIC, MCTS1, TNFAIP8, EZR, UBA1, TXNRD1, FBXO6, BHLHB9, EGFR, MUC1, SOX2, NPM1, HDAC2, LAS1L, PELP1, WASL, VCAM1, MAP1LC3A, ITGA4, FN1, CFTR, RPL7, CA9, NOTCH1, IPO4, TNPO1, KPNB1, RAN, CUL3, ACD, TINF2, TERF1, BKRF1,
Protein Size: 1115

Molecular Weight: 125 kDa

Gene ID: 3843

NCBI Accession: NM_002271, NP_002262

Pathways: Protein targeting to Nucleus