ULK1 antibody (N-Term)

Catalog No. ABIN2785618

This Rabbit Polyclonal antibody specifically detects ULK1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for ULK1 antibody (N-Term) (ABIN2785618)

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ULK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ULK1 Antibody

Binding Specificity: N-Term

Sequence: PEVIMSQHYD GKADLWSIGT IVYQCLTGKA PFQASSPQDL RLFYEKNKTL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ULK1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human ULK1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ULK1

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Alternative Name: ULK1

Background: ULK1 is a serine/threonine-protein kinase involved in autophagy in response to starvation. It acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. It is activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. It may phosphorylate ATG13/KIAA0652 and RPTOR, however such data need additional evidences and plays a role early in neuronal differentiation and is required for granule cell axon formation.
Alias Symbols: ATG1, ATG1A, UNC51, Unc51.1, hATG1
Protein Interaction Partner: SESN2, SQSTM1, ATG16L1, EXOC8, EXOC2, BECN1, RALB, AMBRA1, ULK1, UBC, TRAF6, MBP, ATG13, MAP1LC3C, MAP1LC3A, MAP1LC3B, GABARAPL1, GABARAPL2, GABARAP, MLST8, RPTOR, MTOR, PPP1CA, ATG4B, CDC37, G3BP1, RB1CC1, USP10, PRKAG1, PRKAB2, PRKAA2, PRKAA1, SYNGAP1,
Protein Size: 1050

Molecular Weight: 112 kDa

Gene ID: 8408

NCBI Accession: NM_003565, NP_003556

UniProt: O75385

Pathways: Regulation of Cell Size, Autophagy