GCAP1 antibody (N-Term)

Catalog No. ABIN2785648

Product Details anti-GCAP1 Antibody

Target: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LYEFRQFFGL KNLSPSASQY VEQMFETFDF NKDGYIDFME YVAALSLVLK

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against GUCA1A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GUCA1A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 201 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GCAP1

Target: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Alternative Name: GUCA1A (GUCA1A Products)

Background: GUCA1A(GCAP1) plays a role in the recovery of retinal photoreceptors from photobleaching. In the recovery phase, the phototransduction messeneger cGMP is replenished by retinal guanylyl cyclase-1 (GC1). GC1 is activated by decreasing Ca(2+) concentrations following photobleaching. The protein mediates the sensitivity of GC1 to Ca(2+) concentrations. GCAP1 promotes activity of GC1 at low Ca(2+) concentrations and inhibits GC1 activity at high Ca(2+) concentrations. Mutations in this gene cause autosomal dominant cone dystrophy (COD3), a disease characterized by reduced visual acuity associated with progressive loss of color vision. Mutations in this gene prohibit the inactivation of RetGC1 at high Ca(2+) concentrations, causing the constitutive activation of RetGC1 and, presumably, increased cell death. This gene is expressed in retina and spermatagonia.
Alias Symbols: COD3, GCAP, GCAP1, GUCA, GUCA1, CORD14, C6orf131, dJ139D8.6
Protein Interaction Partner: GUCY2D,
Protein Size: 201

Molecular Weight: 23 kDa

Gene ID: 2978

NCBI Accession: NM_000409, NP_000400

UniProt: P43080

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction