Histone Cluster 1, H1t (HIST1H1T) (Middle Region) antibody

Catalog No. ABIN2785870

Product Details

Target: Histone Cluster 1, H1t (HIST1H1T)

Binding Specificity: Middle Region

Reactivity: Human, Dog, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Sequence: KLSKKVIPKS TRSKAKKSVS AKTKKLVLSR DSKSPKTAKT NKRAKKPRAT

Predicted Reactivity: Dog: 92%, Human: 100%, Pig: 86%, Rabbit: 93%

Characteristics: This is a rabbit polyclonal antibody against HIST1H1T. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HIST1H1T

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 207 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: Histone Cluster 1, H1t (HIST1H1T)

Alternative Name: HIST1H1T (HIST1H1T Products)

Background: Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. HIST1H1T is a member of the histone H1 family. Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Alias Symbols: H1FT, H1t, MGC163222, dJ221C16.2
Protein Interaction Partner: UBC, SUMO1, IRAK4, PRKCA, YWHAQ, POU5F1,
Protein Size: 207

Molecular Weight: 22 kDa

Gene ID: 3010

NCBI Accession: NM_005323, NP_005314

UniProt: P22492