LDLRAP1 antibody (N-Term)

Catalog No. ABIN2786138

Product Details anti-LDLRAP1 Antibody

Target: LDLRAP1 (Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Horse, Pig, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LDLRAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: WTDTRETLLE GMLFSLKYLG MTLVEQPKGE ELSAAAIKRI VATAKASGKK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against LDLRAP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LDLRAP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 308 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for LDLRAP1

Target: LDLRAP1 (Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1))

Alternative Name: LDLRAP1 (LDLRAP1 Products)

Background: LDLRAP1 is an adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). LDLRAP1 may be required for LDL binding and internalization but not for receptor clustering in coated pits. LDLRAP1 may facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. LDLRAP1 may also be involved in the internalization of other LDLR family members. LDLRAP1 binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface.The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: ARH, ARH1, ARH2, DKFZp586D0624, FHCB1, FHCB2, MGC34705
Protein Interaction Partner: OBFC1, AP2B1, UBC, KLC1, LDLR, LRP2, CLTC, TFAP2A, APP,
Protein Size: 308

Molecular Weight: 34 kDa

Gene ID: 26119

NCBI Accession: NM_015627, NP_056442

UniProt: Q5SW96

Pathways: Lipid Metabolism